2020
Sánchez-Rivera, Laura; Santos, Pedro Ferreira; Sevilla, M Angeles; Montero, M José; Recio, Isidra; Miralles, Beatriz
Implication of Opioid Receptors in the Antihypertensive Effect of a Bovine Casein Hydrolysate and α-Casein-Derived Peptides Artículo de revista
En: J Agric Food Chem, vol. 68, no 7, pp. 1877–1883, 2020, ISSN: 1520-5118.
Resumen | Enlaces | BibTeX | Etiquetas: antihypertensive peptide, casein hydrolysate, naloxone, opioid receptor, spontaneously hypertensive rat
@article{pmid31402656,
title = {Implication of Opioid Receptors in the Antihypertensive Effect of a Bovine Casein Hydrolysate and α-Casein-Derived Peptides},
author = {Laura Sánchez-Rivera and Pedro Ferreira Santos and M Angeles Sevilla and M José Montero and Isidra Recio and Beatriz Miralles},
url = {https://pubs.acs.org/doi/10.1021/acs.jafc.9b03872},
doi = {10.1021/acs.jafc.9b03872},
issn = {1520-5118},
year = {2020},
date = {2020-02-01},
urldate = {2020-02-01},
journal = {J Agric Food Chem},
volume = {68},
number = {7},
pages = {1877--1883},
abstract = {The antihypertensive activity of two α-casein-derived peptides and casein hydrolysate containing these sequences was evaluated in the presence of naloxone. The activity was abolished by this opioid antagonist at 2, 4, and 6 h post-administration. Similarly, the antihypertensive effect of the α-casein peptides RYLGY (-23.8 ± 2.5 mmHg) and AYFYPEL (-21.1 ± 3.2 mmHg) at 5 mg/kg of body weight was antagonized by the co-administration of naloxone. Because peptide AYFYPEL had recently shown opioid activity, a molecular dynamic simulation of this peptide with human μ-opioid receptor was performed to demonstrate its favorable structure and interaction energy, despite the presence of Ala at the N terminus. Altogether, these results revealed that the effect on systolic blood pressure of the studied α-casein peptides is mediated by interaction with opioid receptors and the antihypertensive activity of casein hydrolysate can be very likely ascribed to them with the possible contribution of other mechanisms.},
keywords = {antihypertensive peptide, casein hydrolysate, naloxone, opioid receptor, spontaneously hypertensive rat},
pubstate = {published},
tppubtype = {article}
}
The antihypertensive activity of two α-casein-derived peptides and casein hydrolysate containing these sequences was evaluated in the presence of naloxone. The activity was abolished by this opioid antagonist at 2, 4, and 6 h post-administration. Similarly, the antihypertensive effect of the α-casein peptides RYLGY (-23.8 ± 2.5 mmHg) and AYFYPEL (-21.1 ± 3.2 mmHg) at 5 mg/kg of body weight was antagonized by the co-administration of naloxone. Because peptide AYFYPEL had recently shown opioid activity, a molecular dynamic simulation of this peptide with human μ-opioid receptor was performed to demonstrate its favorable structure and interaction energy, despite the presence of Ala at the N terminus. Altogether, these results revealed that the effect on systolic blood pressure of the studied α-casein peptides is mediated by interaction with opioid receptors and the antihypertensive activity of casein hydrolysate can be very likely ascribed to them with the possible contribution of other mechanisms.
2018
Carrillo, Wilman; Monteiro, Karin Maia; Mart'inez-Maqueda, Daniel; Ramos, Mercedes; Recio, Isidra; ao Ernesto de Carvalho, Jo
Antiulcerative Activity of Milk Proteins Hydrolysates Artículo de revista
En: Journal of Medicinal Food, vol. 21, no 4, pp. 408–415, 2018.
Resumen | Enlaces | BibTeX | Etiquetas: antiulcerative activity, casein hydrolysate, peptides, rat model, whey protein hydrolysate
@article{Carrillo2018-ku,
title = {Antiulcerative Activity of Milk Proteins Hydrolysates},
author = {Wilman Carrillo and Karin Maia Monteiro and Daniel Mart'inez-Maqueda and Mercedes Ramos and Isidra Recio and Jo ao Ernesto de Carvalho},
url = {https://www.liebertpub.com/doi/10.1089/jmf.2017.0087},
doi = {https://doi.org/10.1089/jmf.2017.0087},
year = {2018},
date = {2018-04-01},
urldate = {2018-04-01},
journal = {Journal of Medicinal Food},
volume = {21},
number = {4},
pages = {408--415},
publisher = {Mary Ann Liebert, Inc., publishers},
abstract = {Abstract Several studies have shown the protective effect of
dairy products, especially $alpha$-lactalbumin and derived
hydrolysates, against induced gastric ulcerative lesions. The
mucus strengthening represents an important mechanism in the
defense of gastrointestinal mucosa. Previously, a hydrolysate
from casein (CNH) and a hydrolysate from whey protein
concentrate rich in ?-lactoglobulin (WPH) demonstrated a
stimulatory activity on mucus production in intestinal goblet
cells. The aim of this work was to evaluate the possible
antiulcerative activity of these two hydrolysates in an
ethanol-induced ulcer model in rats. All tested samples
significantly reduced the ulcerative lesions index (ULI),
compared with the saline solution, using doses of 300 and
1000?mg kg?1 body weight with decreases up to 66.3% ULI. A
dose?response relationship was found for both hydrolysates. The
involvement of endogenous sulfhydryl (SH) groups and
prostaglandins (PGs) in the antiulcerative activity was
evaluated using their blockage. The antiulcerative activity of
WPH showed a drastic decrease in presence of N-ethylmaleimide
(from 41.4% to 9.2% ULI). However, the CNH antiulcerative
properties were not significantly affected. The cytoprotective
effect of WPH appears to depend on a PG-mediated mechanism. In
conclusion, CNH and WPH demonstrated in vivo antiulcerative
properties and represent a promising alternative as protectors
of the gastric mucosa.},
keywords = {antiulcerative activity, casein hydrolysate, peptides, rat model, whey protein hydrolysate},
pubstate = {published},
tppubtype = {article}
}
Abstract Several studies have shown the protective effect of
dairy products, especially $alpha$-lactalbumin and derived
hydrolysates, against induced gastric ulcerative lesions. The
mucus strengthening represents an important mechanism in the
defense of gastrointestinal mucosa. Previously, a hydrolysate
from casein (CNH) and a hydrolysate from whey protein
concentrate rich in ?-lactoglobulin (WPH) demonstrated a
stimulatory activity on mucus production in intestinal goblet
cells. The aim of this work was to evaluate the possible
antiulcerative activity of these two hydrolysates in an
ethanol-induced ulcer model in rats. All tested samples
significantly reduced the ulcerative lesions index (ULI),
compared with the saline solution, using doses of 300 and
1000?mg kg?1 body weight with decreases up to 66.3% ULI. A
dose?response relationship was found for both hydrolysates. The
involvement of endogenous sulfhydryl (SH) groups and
prostaglandins (PGs) in the antiulcerative activity was
evaluated using their blockage. The antiulcerative activity of
WPH showed a drastic decrease in presence of N-ethylmaleimide
(from 41.4% to 9.2% ULI). However, the CNH antiulcerative
properties were not significantly affected. The cytoprotective
effect of WPH appears to depend on a PG-mediated mechanism. In
conclusion, CNH and WPH demonstrated in vivo antiulcerative
properties and represent a promising alternative as protectors
of the gastric mucosa.
dairy products, especially $alpha$-lactalbumin and derived
hydrolysates, against induced gastric ulcerative lesions. The
mucus strengthening represents an important mechanism in the
defense of gastrointestinal mucosa. Previously, a hydrolysate
from casein (CNH) and a hydrolysate from whey protein
concentrate rich in ?-lactoglobulin (WPH) demonstrated a
stimulatory activity on mucus production in intestinal goblet
cells. The aim of this work was to evaluate the possible
antiulcerative activity of these two hydrolysates in an
ethanol-induced ulcer model in rats. All tested samples
significantly reduced the ulcerative lesions index (ULI),
compared with the saline solution, using doses of 300 and
1000?mg kg?1 body weight with decreases up to 66.3% ULI. A
dose?response relationship was found for both hydrolysates. The
involvement of endogenous sulfhydryl (SH) groups and
prostaglandins (PGs) in the antiulcerative activity was
evaluated using their blockage. The antiulcerative activity of
WPH showed a drastic decrease in presence of N-ethylmaleimide
(from 41.4% to 9.2% ULI). However, the CNH antiulcerative
properties were not significantly affected. The cytoprotective
effect of WPH appears to depend on a PG-mediated mechanism. In
conclusion, CNH and WPH demonstrated in vivo antiulcerative
properties and represent a promising alternative as protectors
of the gastric mucosa.
2017
Bounouala, Fatima Zohra; Roudj, Salima; Karam, Nour-Eddine; Recio, Isidra; Miralles, Beatriz
Casein Hydrolysates by Lactobacillus brevis and Lactococcus lactis Proteases: Peptide Profile Discriminates Strain-Dependent Enzyme Specificity Artículo de revista
En: Journal of Agricultural and Food Chemistry, vol. 65, no 42, pp. 9324-9332, 2017, ISSN: 0021-8561.
Enlaces | BibTeX | Etiquetas: bioactive peptides, casein hydrolysate, enzyme specificity, lactic acid bacteria, QSAR, sheep milk, Tandem mass spectrometry
@article{Bounouala2017,
title = {Casein Hydrolysates by Lactobacillus brevis and Lactococcus lactis Proteases: Peptide Profile Discriminates Strain-Dependent Enzyme Specificity},
author = {Fatima Zohra Bounouala and Salima Roudj and Nour-Eddine Karam and Isidra Recio and Beatriz Miralles},
url = {https://doi.org/10.1021/acs.jafc.7b03203},
doi = {10.1021/acs.jafc.7b03203},
issn = {0021-8561},
year = {2017},
date = {2017-10-25},
urldate = {2017-10-25},
journal = {Journal of Agricultural and Food Chemistry},
volume = {65},
number = {42},
pages = {9324-9332},
publisher = {American Chemical Society},
keywords = {bioactive peptides, casein hydrolysate, enzyme specificity, lactic acid bacteria, QSAR, sheep milk, Tandem mass spectrometry},
pubstate = {published},
tppubtype = {article}
}
Fernández-Tomé, Samuel; Martínez-Maqueda, Daniel; Tabernero, María; Largo, Carlota; Recio, Isidra; Miralles, Beatriz
Effect of the long-term intake of a casein hydrolysate on mucin secretion and gene expression in the rat intestine Artículo de revista
En: Journal of Functional Foods, vol. 33, pp. 176-180, 2017, ISSN: 1756-4646.
Resumen | Enlaces | BibTeX | Etiquetas: casein hydrolysate, Faecal mucin, Gene expression, O-linked glycoprotein, Rat intestine
@article{FERNANDEZTOME2017176,
title = {Effect of the long-term intake of a casein hydrolysate on mucin secretion and gene expression in the rat intestine},
author = {Samuel Fernández-Tomé and Daniel Martínez-Maqueda and María Tabernero and Carlota Largo and Isidra Recio and Beatriz Miralles},
url = {https://www.sciencedirect.com/science/article/pii/S1756464617301536},
doi = {https://doi.org/10.1016/j.jff.2017.03.036},
issn = {1756-4646},
year = {2017},
date = {2017-01-01},
urldate = {2017-01-01},
journal = {Journal of Functional Foods},
volume = {33},
pages = {176-180},
abstract = {The aim of the present study was to investigate the in vivo effect on rat intestinal mucus production of a casein hydrolysate that had been previously shown a mucin stimulatory effect in human goblet cells (HT29-MTX). A significant rise of O-linked glycoproteins in faeces after 2 and 8weeks of the casein hydrolysate intake was observed. In agreement with this increased secretion, the relative expression for the genes that encode the secreted MUC2 and the membrane-bound MUC3 was significantly increased in ileum and colon. Mucus material in the small intestine lumen of rats fed the casein hydrolysate was higher than that found in control rats, however the change did not reach statistical difference. This study confirms in vivo the potential effect on mucus gastrointestinal protection of this casein hydrolysate.},
keywords = {casein hydrolysate, Faecal mucin, Gene expression, O-linked glycoprotein, Rat intestine},
pubstate = {published},
tppubtype = {article}
}
The aim of the present study was to investigate the in vivo effect on rat intestinal mucus production of a casein hydrolysate that had been previously shown a mucin stimulatory effect in human goblet cells (HT29-MTX). A significant rise of O-linked glycoproteins in faeces after 2 and 8weeks of the casein hydrolysate intake was observed. In agreement with this increased secretion, the relative expression for the genes that encode the secreted MUC2 and the membrane-bound MUC3 was significantly increased in ileum and colon. Mucus material in the small intestine lumen of rats fed the casein hydrolysate was higher than that found in control rats, however the change did not reach statistical difference. This study confirms in vivo the potential effect on mucus gastrointestinal protection of this casein hydrolysate.
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