Publicaciones

@article{FONTESCANDIA2022107505,

title = {Development of polysaccharide-casein gel-like structures resistant to in vitro gastric digestion},

author = {Cynthia Fontes-Candia and Pablo Jiménez-Barrios and Beatriz Miralles and Isidra Recio and Amparo López-Rubio and Marta Martínez-Sanz},

url = {https://www.sciencedirect.com/science/article/pii/S0268005X2200025X},

doi = {https://doi.org/10.1016/j.foodhyd.2022.107505},

issn = {0268-005X},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Food Hydrocolloids},

volume = {127},

pages = {107505},

abstract = {Controlling protein digestion is a promising strategy to modulate hormonal responses involved in satiety and appetite regulation. In this context, polysaccharide-casein gel-like structures have been developed and subjected to in-vitro gastrointestinal digestions to evaluate their potential for delaying casein hydrolysis. The effect of the polysaccharide type (agar vs. κ-carrageenan), the polysaccharide:casein ratio and the physical state of the structures (hydrogels vs. aerogels) on the protection ability was investigated. The microstructure evolution of the materials upon the digestions was studied and the molecular weight distribution and peptidomic profile of the digestion products were also determined. During the gastric phase most of the developed structures exerted a protective effect and intact casein clusters were even detected in some of the formulations. In contrast, during the intestinal phase most of the casein was released and hydrolysed to a certain extent. In general, the hydrogels showed a greater protective effect than the aerogels, due to a limited diffusion of the protein towards the liquid medium. Moreover, a higher polysaccharide:protein ratio produced stronger gel networks which provided greater protection. In particular, agar-based and κ-carrageenan hydrogels with 25% polysaccharide and agar-based aerogels with 75% polysaccharide would be the most optimum for delaying casein digestion, since they were able to preserve intact casein after the gastric phase while promoting the release of peptides during the intestinal phase.},

keywords = {Aerogels, bioactive proteins, body mass index, Controlled digestibility, Human milk, Hydrogels, in vitro infant digestion, MALDI mass spectrometry, Simulated gastrointestinal digestion, Sulphated polysaccharides},

pubstate = {published},

tppubtype = {article}

}

@article{foods10040887,

title = {Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers},

author = {Sánchez-Hernández, Silvia and Théron, Laëtitia and Jiménez-Barrios, Pablo and Olalla-Herrera, Manuel and Isidra Recio and Beatriz Miralles},

url = {https://www.mdpi.com/2304-8158/10/4/887},

doi = {https://doi.org/110.3390/foods10040887},

issn = {2304-8158},

year  = {2021},

date = {2021-04-18},

urldate = {2021-04-18},

journal = {Foods},

volume = {10},

abstract = {Human milk proteins have shown to vary in concentration and distribution through lactation. However, while some regulatory components, such as hormones, have shown associations with regard to the mothers’ body mass index, there is limited information on the possible influence of this condition on the whole protein distribution. The objective of this study was to evaluate the protein profile of human milk from normal weight and overweight or obese mothers to identify differences in protein expression in colostrum, transitional and mature milk. The mass spectrometry analysis showed the ability to class with a high degree of confidence the lactation state and the milk profile according to the mother’s condition. Individual milk samples were subjected to a digestion in vitro model that takes into account the specificities of the gastrointestinal conditions of full-term newborn infants. The digestion products were compared with available data from the digestive contents in newborns. The behavior of the most abundant proteins and the overall peptide generation and survival, showed good correspondence with in vivo data.},

keywords = {bioactive proteins, body mass index, Human milk, in vitro infant digestion, MALDI mass spectrometry},

pubstate = {published},

tppubtype = {article}

}

@article{SANCHEZHERNANDEZ2021110149,

title = {Study of the phenolic compound profile and antioxidant activity of human milk from Spanish women at different stages of lactation: A comparison with infant formulas},

author = {Silvia Sánchez-Hernández and Adelaida Esteban-Muñoz and Cristina Samaniego-Sánchez and Rafael Giménez-Martínez and Beatriz Miralles and Manuel Olalla-Herrera},

url = {https://www.sciencedirect.com/science/article/pii/S0963996921000466},

doi = {https://doi.org/10.1016/j.foodres.2021.110149},

issn = {0963-9969},

year  = {2021},

date = {2021-01-01},

journal = {Food Research International},

volume = {141},

pages = {110149},

abstract = {Human milk (HM) has been proven to have important and essential antioxidant properties to counteract infant susceptibility to oxidative stress. Phenolic compounds are secondary metabolites which come from plants and are potent natural antioxidants. The ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method used in the present study allowed the quantification of 26 phenolic compounds (ten hydroxybenzoic acids, seven hydroxycinnamic acids, four flavonoids, three hydroxybenzaldehydes and two other polyphenols) in HM samples at different stages of lactation (colostrum, transitional milk and mature milk) and infant formulas (IF). Many of the phenolic compounds identified have been reported to be present in HM for the first time. The total phenolic compound content (TPC) was quantified using the Folin assay and the antioxidant activity (AC) was evaluated with the DPPH, ABTS and FRAP assays. Significant differences were evidenced between HM and IF. HM from mothers with an adherence to a Mediterranean diet contained twice as many individual phenolic compounds as infant formulas, with a higher proportion of hydroxybenzoic acids. Conversely, IF showed a higher proportion of hydroxycinnamic acids. Overall, the antioxidant activity of HM showed small variations during lactation.},

keywords = {Antioxidant activity, Human milk, Hydroxybenzoic acids, Hydroxycinnamic acids, Infant formulas, Phenolic acids, Phenolic compounds},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31847315,

title = {A Comparison of Changes in the Fatty Acid Profile of Human Milk of Spanish Lactating Women during the First Month of Lactation Using Gas Chromatography-Mass Spectrometry. A Comparison with Infant Formulas},

author = {Silvia Sánchez-Hernández and Adelaida Esteban-Muñoz and Rafael Giménez-Martínez and María José Aguilar-Cordero and Beatriz Miralles and Manuel Olalla-Herrera},

url = {https://www.mdpi.com/2072-6643/11/12/3055},

doi = {10.3390/nu11123055},

issn = {2072-6643},

year  = {2019},

date = {2019-12-01},

urldate = {2019-12-01},

journal = {Nutrients},

volume = {11},

number = {12},

abstract = {Breastfeeding is the ideal way to provide infants with the nutrients they need for healthy growth and development. Milk composition changes throughout lactation, and fat is one of the most variable nutrients in human milk. The aim of this study was to determine the main differences between the fatty acid (FA) profile of human milk samples (colostrum, transitional, and mature milk group) and infant formulas. Human milk samples were provided by lactating women from Granada. Moreover, different commercial infant formulas were analyzed. FAs were determined using gas chromatography coupled with mass spectrometry. According to the results, oleic acid was the predominant monounsaturated fatty acid (41.93% in human milk and 43.53% in infant formulas), while palmitic acid was the most representative saturated fatty acid (20.88% in human milk and 23.09% in infant formulas). Significant differences were found between human milk groups and infant formulas, mainly in long-chain polyunsaturated FAs (LC-PUFAs). The content of araquidonic acid (AA) and docoxahexaenoic acid (DHA) was higher in human milk (0.51% and 0.39%, respectively) than in infant formulas (0.31% and 0.22%, respectively). Linoleic acid (LA) percentage (15.31%) in infant formulas was similar to that found in human milk (14.6%). However, α-linolenic acid (ALA) values were also much higher in infant formulas than in human milk (1.64% and 0.42%, respectively).},

keywords = {fatty acids, GC-MS/MS, Human milk, Infant formulas, LC-PUFA},

pubstate = {published},

tppubtype = {article}

}