2022
Fontes-Candia, Cynthia; Jiménez-Barrios, Pablo; Miralles, Beatriz; Recio, Isidra; López-Rubio, Amparo; Martínez-Sanz, Marta
Development of polysaccharide-casein gel-like structures resistant to in vitro gastric digestion Artículo de revista
En: Food Hydrocolloids, vol. 127, pp. 107505, 2022, ISSN: 0268-005X.
Resumen | Enlaces | BibTeX | Etiquetas: Aerogels, bioactive proteins, body mass index, Controlled digestibility, Human milk, Hydrogels, in vitro infant digestion, MALDI mass spectrometry, Simulated gastrointestinal digestion, Sulphated polysaccharides
@article{FONTESCANDIA2022107505,
title = {Development of polysaccharide-casein gel-like structures resistant to in vitro gastric digestion},
author = {Cynthia Fontes-Candia and Pablo Jiménez-Barrios and Beatriz Miralles and Isidra Recio and Amparo López-Rubio and Marta Martínez-Sanz},
url = {https://www.sciencedirect.com/science/article/pii/S0268005X2200025X},
doi = {https://doi.org/10.1016/j.foodhyd.2022.107505},
issn = {0268-005X},
year = {2022},
date = {2022-01-01},
urldate = {2022-01-01},
journal = {Food Hydrocolloids},
volume = {127},
pages = {107505},
abstract = {Controlling protein digestion is a promising strategy to modulate hormonal responses involved in satiety and appetite regulation. In this context, polysaccharide-casein gel-like structures have been developed and subjected to in-vitro gastrointestinal digestions to evaluate their potential for delaying casein hydrolysis. The effect of the polysaccharide type (agar vs. κ-carrageenan), the polysaccharide:casein ratio and the physical state of the structures (hydrogels vs. aerogels) on the protection ability was investigated. The microstructure evolution of the materials upon the digestions was studied and the molecular weight distribution and peptidomic profile of the digestion products were also determined. During the gastric phase most of the developed structures exerted a protective effect and intact casein clusters were even detected in some of the formulations. In contrast, during the intestinal phase most of the casein was released and hydrolysed to a certain extent. In general, the hydrogels showed a greater protective effect than the aerogels, due to a limited diffusion of the protein towards the liquid medium. Moreover, a higher polysaccharide:protein ratio produced stronger gel networks which provided greater protection. In particular, agar-based and κ-carrageenan hydrogels with 25% polysaccharide and agar-based aerogels with 75% polysaccharide would be the most optimum for delaying casein digestion, since they were able to preserve intact casein after the gastric phase while promoting the release of peptides during the intestinal phase.},
keywords = {Aerogels, bioactive proteins, body mass index, Controlled digestibility, Human milk, Hydrogels, in vitro infant digestion, MALDI mass spectrometry, Simulated gastrointestinal digestion, Sulphated polysaccharides},
pubstate = {published},
tppubtype = {article}
}
Controlling protein digestion is a promising strategy to modulate hormonal responses involved in satiety and appetite regulation. In this context, polysaccharide-casein gel-like structures have been developed and subjected to in-vitro gastrointestinal digestions to evaluate their potential for delaying casein hydrolysis. The effect of the polysaccharide type (agar vs. κ-carrageenan), the polysaccharide:casein ratio and the physical state of the structures (hydrogels vs. aerogels) on the protection ability was investigated. The microstructure evolution of the materials upon the digestions was studied and the molecular weight distribution and peptidomic profile of the digestion products were also determined. During the gastric phase most of the developed structures exerted a protective effect and intact casein clusters were even detected in some of the formulations. In contrast, during the intestinal phase most of the casein was released and hydrolysed to a certain extent. In general, the hydrogels showed a greater protective effect than the aerogels, due to a limited diffusion of the protein towards the liquid medium. Moreover, a higher polysaccharide:protein ratio produced stronger gel networks which provided greater protection. In particular, agar-based and κ-carrageenan hydrogels with 25% polysaccharide and agar-based aerogels with 75% polysaccharide would be the most optimum for delaying casein digestion, since they were able to preserve intact casein after the gastric phase while promoting the release of peptides during the intestinal phase.
2021
Sánchez-Hernández, Silvia; Théron, Laëtitia; Jiménez-Barrios, Pablo; Olalla-Herrera, Manuel; Recio, Isidra; Miralles, Beatriz
Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers Artículo de revista
En: Foods, vol. 10, 2021, ISSN: 2304-8158.
Resumen | Enlaces | BibTeX | Etiquetas: bioactive proteins, body mass index, Human milk, in vitro infant digestion, MALDI mass spectrometry
@article{foods10040887,
title = {Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers},
author = {Sánchez-Hernández, Silvia and Théron, Laëtitia and Jiménez-Barrios, Pablo and Olalla-Herrera, Manuel and Isidra Recio and Beatriz Miralles},
url = {https://www.mdpi.com/2304-8158/10/4/887},
doi = {https://doi.org/110.3390/foods10040887},
issn = {2304-8158},
year = {2021},
date = {2021-04-18},
urldate = {2021-04-18},
journal = {Foods},
volume = {10},
abstract = {Human milk proteins have shown to vary in concentration and distribution through lactation. However, while some regulatory components, such as hormones, have shown associations with regard to the mothers’ body mass index, there is limited information on the possible influence of this condition on the whole protein distribution. The objective of this study was to evaluate the protein profile of human milk from normal weight and overweight or obese mothers to identify differences in protein expression in colostrum, transitional and mature milk. The mass spectrometry analysis showed the ability to class with a high degree of confidence the lactation state and the milk profile according to the mother’s condition. Individual milk samples were subjected to a digestion in vitro model that takes into account the specificities of the gastrointestinal conditions of full-term newborn infants. The digestion products were compared with available data from the digestive contents in newborns. The behavior of the most abundant proteins and the overall peptide generation and survival, showed good correspondence with in vivo data.},
keywords = {bioactive proteins, body mass index, Human milk, in vitro infant digestion, MALDI mass spectrometry},
pubstate = {published},
tppubtype = {article}
}
Human milk proteins have shown to vary in concentration and distribution through lactation. However, while some regulatory components, such as hormones, have shown associations with regard to the mothers’ body mass index, there is limited information on the possible influence of this condition on the whole protein distribution. The objective of this study was to evaluate the protein profile of human milk from normal weight and overweight or obese mothers to identify differences in protein expression in colostrum, transitional and mature milk. The mass spectrometry analysis showed the ability to class with a high degree of confidence the lactation state and the milk profile according to the mother’s condition. Individual milk samples were subjected to a digestion in vitro model that takes into account the specificities of the gastrointestinal conditions of full-term newborn infants. The digestion products were compared with available data from the digestive contents in newborns. The behavior of the most abundant proteins and the overall peptide generation and survival, showed good correspondence with in vivo data.
© GRUPO DE PROTEÍNAS ALIMENTARIAS - 