Publicaciones

@article{SANCHEZHERNANDEZ2021110149,

title = {Study of the phenolic compound profile and antioxidant activity of human milk from Spanish women at different stages of lactation: A comparison with infant formulas},

author = {Silvia Sánchez-Hernández and Adelaida Esteban-Muñoz and Cristina Samaniego-Sánchez and Rafael Giménez-Martínez and Beatriz Miralles and Manuel Olalla-Herrera},

url = {https://www.sciencedirect.com/science/article/pii/S0963996921000466},

doi = {https://doi.org/10.1016/j.foodres.2021.110149},

issn = {0963-9969},

year  = {2021},

date = {2021-01-01},

journal = {Food Research International},

volume = {141},

pages = {110149},

abstract = {Human milk (HM) has been proven to have important and essential antioxidant properties to counteract infant susceptibility to oxidative stress. Phenolic compounds are secondary metabolites which come from plants and are potent natural antioxidants. The ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method used in the present study allowed the quantification of 26 phenolic compounds (ten hydroxybenzoic acids, seven hydroxycinnamic acids, four flavonoids, three hydroxybenzaldehydes and two other polyphenols) in HM samples at different stages of lactation (colostrum, transitional milk and mature milk) and infant formulas (IF). Many of the phenolic compounds identified have been reported to be present in HM for the first time. The total phenolic compound content (TPC) was quantified using the Folin assay and the antioxidant activity (AC) was evaluated with the DPPH, ABTS and FRAP assays. Significant differences were evidenced between HM and IF. HM from mothers with an adherence to a Mediterranean diet contained twice as many individual phenolic compounds as infant formulas, with a higher proportion of hydroxybenzoic acids. Conversely, IF showed a higher proportion of hydroxycinnamic acids. Overall, the antioxidant activity of HM showed small variations during lactation.},

keywords = {Antioxidant activity, Human milk, Hydroxybenzoic acids, Hydroxycinnamic acids, Infant formulas, Phenolic acids, Phenolic compounds},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31847315,

title = {A Comparison of Changes in the Fatty Acid Profile of Human Milk of Spanish Lactating Women during the First Month of Lactation Using Gas Chromatography-Mass Spectrometry. A Comparison with Infant Formulas},

author = {Silvia Sánchez-Hernández and Adelaida Esteban-Muñoz and Rafael Giménez-Martínez and María José Aguilar-Cordero and Beatriz Miralles and Manuel Olalla-Herrera},

url = {https://www.mdpi.com/2072-6643/11/12/3055},

doi = {10.3390/nu11123055},

issn = {2072-6643},

year  = {2019},

date = {2019-12-01},

urldate = {2019-12-01},

journal = {Nutrients},

volume = {11},

number = {12},

abstract = {Breastfeeding is the ideal way to provide infants with the nutrients they need for healthy growth and development. Milk composition changes throughout lactation, and fat is one of the most variable nutrients in human milk. The aim of this study was to determine the main differences between the fatty acid (FA) profile of human milk samples (colostrum, transitional, and mature milk group) and infant formulas. Human milk samples were provided by lactating women from Granada. Moreover, different commercial infant formulas were analyzed. FAs were determined using gas chromatography coupled with mass spectrometry. According to the results, oleic acid was the predominant monounsaturated fatty acid (41.93% in human milk and 43.53% in infant formulas), while palmitic acid was the most representative saturated fatty acid (20.88% in human milk and 23.09% in infant formulas). Significant differences were found between human milk groups and infant formulas, mainly in long-chain polyunsaturated FAs (LC-PUFAs). The content of araquidonic acid (AA) and docoxahexaenoic acid (DHA) was higher in human milk (0.51% and 0.39%, respectively) than in infant formulas (0.31% and 0.22%, respectively). Linoleic acid (LA) percentage (15.31%) in infant formulas was similar to that found in human milk (14.6%). However, α-linolenic acid (ALA) values were also much higher in infant formulas than in human milk (1.64% and 0.42%, respectively).},

keywords = {fatty acids, GC-MS/MS, Human milk, Infant formulas, LC-PUFA},

pubstate = {published},

tppubtype = {article}

}

@article{GOMEZGALLEGO2016924,

title = {Effect of processing on polyamine content and bioactive peptides released after in vitro gastrointestinal digestion of infant formulas},

author = {C. Gómez-Gallego and Isidra Recio and V. Gómez-Gómez and I. Ortuño and M. J. Bernal and G. Ros and M. J. Periago},

url = {https://www.journalofdairyscience.org/article/S0022-0302(15)00890-5/fulltext},

doi = {https://doi.org/10.3168/jds.2015-10030},

issn = {0022-0302},

year  = {2016},

date = {2016-01-01},

urldate = {2016-01-01},

journal = {Journal of Dairy Science},

volume = {99},

number = {2},

pages = {924-932},

abstract = {This study examined the influence of processing on polyamines and peptide release after the digestion of a commercial infant formula designed for children during the first months of life. Polyamine oxidase activity was not suppressed during the manufacturing process, which implicates that polyamine concentrations were reduced over time and during infant formula self-life. In gel electrophoresis, in vitro gastrointestinal digestion of samples with reduced amount of enzymes and time of digestion shows an increase in protein digestibility, reflected in the increase in nonprotein nitrogen after digestion and the disappearance of β-lactoglobulin and α-lactalbumin bands in gel electrophoresis. Depending on the sample, between 22 and 87 peptides were identified after gastrointestinal digestion. A peptide from β-casein f(98–105) with the sequence VKEAMAPK and antioxidant activity appeared in all of the samples. Other peptides with antioxidant, immunomodulatory, and antimicrobial activities were frequently found, which could have an effect on infant health. The present study confirms that the infant formula manufacturing process determines the polyamine content and peptidic profile after digestion of the infant formula. Because compositional dissimilarity between human milk and infant formula in polyamines and proteins could be responsible for some of the differences in health reported between breast-fed and formula-fed children, these changes must be taken into consideration because they may have a great effect on infant nutrition and development.},

keywords = {Infant formulas, Mass spectrometry, peptide, polyamine, Simulated gastrointestinal digestion},

pubstate = {published},

tppubtype = {article}

}