2016
Egger, Lotti; Ménard, Olivia; Delgado-Andrade, Cristina; Alvito, Paula; Assunção, Ricardo; Balance, Simon; Barberá, Reyes; Brodkorb, Andre; Cattenoz, Thomas; Clemente, Alfonso; Comi, Irene; Dupont, Didier; Garcia-Llatas, Guadalupe; Lagarda, María Jesús; Feunteun, Steven Le; JanssenDuijghuijsen, Lonneke; Karakaya, Sibel; Lesmes, Uri; Mackie, Alan R.; Martins, Carla; Meynier, Anne; Miralles, Beatriz; Murray, Brent S.; Pihlanto, Anne; Picariello, Gianluca; Santos, Claudia N.; Simsek, Sebnem; Recio, Isidra; Rigby, Neil; Rioux, Laurie-Eve; Stoffers, Helena; Tavares, Ana; Tavares, Lucelia; Turgeon, Sylvie; Ulleberg, Ellen K.; Vegarud, Gerd E.; Vergères, Guy; Portmann, Reto
The harmonized INFOGEST in vitro digestion method: From knowledge to action Artículo de revista
En: Food Research International, vol. 88, pp. 217-225, 2016, ISSN: 0963-9969, (The 4th International Conference on Food Digestion).
Resumen | Enlaces | BibTeX | Etiquetas: Dairy proteins, Digestion, Harmonized IVD protocol, Inter-laboratory trial, Mass spectrometry, peptides
@article{EGGER2016217,
title = {The harmonized INFOGEST in vitro digestion method: From knowledge to action},
author = {Lotti Egger and Olivia Ménard and Cristina Delgado-Andrade and Paula Alvito and Ricardo Assunção and Simon Balance and Reyes Barberá and Andre Brodkorb and Thomas Cattenoz and Alfonso Clemente and Irene Comi and Didier Dupont and Guadalupe Garcia-Llatas and María Jesús Lagarda and Steven Le Feunteun and Lonneke JanssenDuijghuijsen and Sibel Karakaya and Uri Lesmes and Alan R. Mackie and Carla Martins and Anne Meynier and Beatriz Miralles and Brent S. Murray and Anne Pihlanto and Gianluca Picariello and Claudia N. Santos and Sebnem Simsek and Isidra Recio and Neil Rigby and Laurie-Eve Rioux and Helena Stoffers and Ana Tavares and Lucelia Tavares and Sylvie Turgeon and Ellen K. Ulleberg and Gerd E. Vegarud and Guy Vergères and Reto Portmann},
url = {https://www.sciencedirect.com/science/article/pii/S0963996915302751},
doi = {https://doi.org/10.1016/j.foodres.2015.12.006},
issn = {0963-9969},
year = {2016},
date = {2016-01-01},
journal = {Food Research International},
volume = {88},
pages = {217-225},
abstract = {Within the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skim milk powder (SMP) as a model food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, the degree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas β-lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase. The study also showed that a few critical steps were responsible for the remaining inter-laboratory variability. The largest deviations arose from the determination of pepsin activity. Therefore, this step was further clarified, harmonized, and implemented in a third inter-laboratory study. The present work gives an overview of all three inter-laboratory studies, showing that the IVD INFOGEST method has led to an increased consistency that enables a better comparability of in vitro digestion studies in the future.},
note = {The 4th International Conference on Food Digestion},
keywords = {Dairy proteins, Digestion, Harmonized IVD protocol, Inter-laboratory trial, Mass spectrometry, peptides},
pubstate = {published},
tppubtype = {article}
}
Within the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skim milk powder (SMP) as a model food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, the degree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas β-lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase. The study also showed that a few critical steps were responsible for the remaining inter-laboratory variability. The largest deviations arose from the determination of pepsin activity. Therefore, this step was further clarified, harmonized, and implemented in a third inter-laboratory study. The present work gives an overview of all three inter-laboratory studies, showing that the IVD INFOGEST method has led to an increased consistency that enables a better comparability of in vitro digestion studies in the future.
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