Publicaciones

@article{VIVANCOMAROTO2022111238,

title = {In vitro digestion of milk proteins including intestinal brush border membrane peptidases. Transepithelial transport of resistant casein domains},

author = {Santiaga María Vivanco-Maroto and Marta Santos-Hernández and Javier Sanchón and Gianluca Picariello and Isidra Recio and Beatriz Miralles},

url = {https://www.sciencedirect.com/science/article/pii/S0963996922002952},

doi = {https://doi.org/10.1016/j.foodres.2022.111238},

issn = {0963-9969},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Food Research International},

volume = {157},

pages = {111238},

abstract = {The use of enzymes from the brush border membrane (BBM) in simulated gastrointestinal digestion of milk proteins has been evaluated. With this purpose, the resistant sequences from casein and milk whey proteins after INFOGEST in vitro digestion with and without BBM have been analyzed by tandem mass spectrometry. The use of BBM revealed additional cleavages to those found with pancreatic enzymes, although the number of total identified peptides decreased due to the reduction of the peptide size. These new cleavages were mainly attributed to the activity of amino- and carboxy-peptidases, which was also reflected in the higher concentration of free amino acids found in the gastrointestinal digests with BBM. The peptidome of the simulated gastrointestinal digests was compared with that previously obtained in digests aspirated from human jejunum after oral administration of the same substrates. The addition of BBM did not change significantly the peptide profile, although it allowed the identification of peptides found in human digests. However, none of the models was able to reproduce the large variety of peptides found in vivo. In addition, in vitro transepithelial transport of six β-casein derived peptides resistant to gastrointestinal digestion, including the opioid β-casomorphin-7, was also evaluated. The results point to the importance of the nature of the N- and C-terminal end for the transport rate through the Caco-2 cell monolayer. Therefore, the use of BBM as a supplementary step after simulated pancreatic digestion can be considered in bioavailability studies since the final sequence can determine the absorption of peptides.},

keywords = {Absorption, Brush border membrane, Caco-2 cell, Casein, Mass spectrometry, Peptidomics, Whey},

pubstate = {published},

tppubtype = {article}

}

@article{GILSANCHEZ201964,

title = {Some new findings on the potential use of biocompatible silver nanoparticles in winemaking},

author = {Irene Gil-Sánchez and Miguel Monge and Beatriz Miralles and Gloria Armentia and Carolina Cueva and Julian Crespo and Jose M. López Luzuriaga and M. Elena Olmos and Begoña Bartolomé and Dolores González Llano and M Victoria Moreno-Arribas},

url = {https://www.sciencedirect.com/science/article/pii/S1466856418300195},

doi = {https://doi.org/10.1016/j.ifset.2018.04.017},

issn = {1466-8564},

year  = {2019},

date = {2019-01-01},

urldate = {2019-01-01},

journal = {Innovative Food Science & Emerging Technologies},

volume = {51},

pages = {64-72},

abstract = {There is currently an increasing commercial demand for silver nanoparticles (Ag NPs) due to their wide applicability in various markets. Because of their powerful antimicrobial properties, these nanoparticles are frequently used for food-associated consumer products. In this paper, the effect of two Ag NPs coated with biocompatible materials – PEG-Ag NPs 20 (polyethylene glycol) and GSH-Ag NPs (reduced glutathione) – to control microbial growth in wines was assessed. Both Ag NPs were subjected to an in vitro three-step digestion, and changes in their morphology and an assessment of their cytotoxicity against Caco-2 cells were determined. Both Ag NPs were effective against the different microbial population present in tested wines. Regarding their in vitro digestion, the size and shape of the nanoparticles were almost unaltered in the case of GSH-Ag NPs, while in PEG-Ag NPs 20 some particle agglomeration was observed. Overall, these results suggest that Ag NPs may reach the intestine in a nano-scaled form. Finally, Caco-2 cell experiments seemed to exclude toxicity of Ag NPs at the intestinal epithelium. 

Industrial relevance 

Traditionally, sulphur dioxide (SO2) has been used by oenologists to control the microbial population in wine. As a result of increasing evidence of possible health risks associated with this additive in wine, there has been growing interest in finding new alternatives to replace it. Silver nanoparticles display a broad spectrum of antimicrobial activity, so they could constitute a very promising approach to reducing SO2 in winemaking.},

note = {Innovations in Food Science and Technology at the Spanish National Research Council (CSIC)},

keywords = {Caco-2 cell, Digestion, Silver nanoparticles, Sulphur dioxide alternatives, Wine bacteria},

pubstate = {published},

tppubtype = {article}

}