2019
Brodkorb, André; Egger, Lotti; Alminger, Marie; Alvito, Paula; c ao, Ricardo Assunc; Ballance, Simon; Bohn, Torsten; Bourlieu-Lacanal, Claire; Boutrou, Rachel; Carri`ere, Frédéric; Clemente, Alfonso; Corredig, Milena; Dupont, Didier; Dufour, Claire; Edwards, Cathrina; Golding, Matt; Karakaya, Sibel; Kirkhus, Bente; Feunteun, Steven Le; Lesmes, Uri; Macierzanka, Adam; Mackie, Alan R; Martins, Carla; Marze, Sébastien; McClements, David Julian; Ménard, Olivia; Minekus, Mans; Portmann, Reto; Santos, Cláudia N; Souchon, Isabelle; Singh, R Paul; Vegarud, Gerd E; Wickham, Martin S J; Weitschies, Werner; Recio, Isidra
INFOGEST static in vitro simulation of gastrointestinal food digestion Artículo de revista
En: Nature Protocols, vol. 14, no 4, pp. 991–1014, 2019, ISSN: 1750-2799.
Resumen | Enlaces | BibTeX | Etiquetas: Biochemistry, Enzymes, Gastrointestinal models, Metabolism
@article{Brodkorb2019-lb,
title = {INFOGEST static in vitro simulation of gastrointestinal food digestion},
author = {André Brodkorb and Lotti Egger and Marie Alminger and Paula Alvito and Ricardo Assunc c ao and Simon Ballance and Torsten Bohn and Claire Bourlieu-Lacanal and Rachel Boutrou and Frédéric Carri`ere and Alfonso Clemente and Milena Corredig and Didier Dupont and Claire Dufour and Cathrina Edwards and Matt Golding and Sibel Karakaya and Bente Kirkhus and Steven Le Feunteun and Uri Lesmes and Adam Macierzanka and Alan R Mackie and Carla Martins and Sébastien Marze and David Julian McClements and Olivia Ménard and Mans Minekus and Reto Portmann and Cláudia N Santos and Isabelle Souchon and R Paul Singh and Gerd E Vegarud and Martin S J Wickham and Werner Weitschies and Isidra Recio},
url = {https://www.nature.com/articles/s41596-018-0119-1},
doi = {https://doi.org/10.1038/s41596-018-0119-1},
issn = {1750-2799},
year = {2019},
date = {2019-04-01},
urldate = {2019-04-01},
journal = {Nature Protocols},
volume = {14},
number = {4},
pages = {991--1014},
abstract = {Developing a mechanistic understanding of the impact of food
structure and composition on human health has increasingly
involved simulating digestion in the upper gastrointestinal
tract. These simulations have used a wide range of different
conditions that often have very little physiological relevance,
and this impedes the meaningful comparison of results. The
standardized protocol presented here is based on an international
consensus developed by the COST INFOGEST network. The method is
designed to be used with standard laboratory equipment and
requires limited experience to encourage a wide range of
researchers to adopt it. It is a static digestion method that
uses constant ratios of meal to digestive fluids and a constant
pH for each step of digestion. This makes the method simple to
use but not suitable for simulating digestion kinetics. Using
this method, food samples are subjected to sequential oral,
gastric and intestinal digestion while parameters such as
electrolytes, enzymes, bile, dilution, pH and time of digestion
are based on available physiological data. This amended and
improved digestion method (INFOGEST 2.0) avoids challenges
associated with the original method, such as the inclusion of the
oral phase and the use of gastric lipase. The method can be used
to assess the endpoints resulting from digestion of foods by
analyzing the digestion products (e.g., peptides/amino acids,
fatty acids, simple sugars) and evaluating the release of
micronutrients from the food matrix. The whole protocol can be
completed in ~7 d, including ~5 d required for the determination
of enzyme activities.},
keywords = {Biochemistry, Enzymes, Gastrointestinal models, Metabolism},
pubstate = {published},
tppubtype = {article}
}
Developing a mechanistic understanding of the impact of food
structure and composition on human health has increasingly
involved simulating digestion in the upper gastrointestinal
tract. These simulations have used a wide range of different
conditions that often have very little physiological relevance,
and this impedes the meaningful comparison of results. The
standardized protocol presented here is based on an international
consensus developed by the COST INFOGEST network. The method is
designed to be used with standard laboratory equipment and
requires limited experience to encourage a wide range of
researchers to adopt it. It is a static digestion method that
uses constant ratios of meal to digestive fluids and a constant
pH for each step of digestion. This makes the method simple to
use but not suitable for simulating digestion kinetics. Using
this method, food samples are subjected to sequential oral,
gastric and intestinal digestion while parameters such as
electrolytes, enzymes, bile, dilution, pH and time of digestion
are based on available physiological data. This amended and
improved digestion method (INFOGEST 2.0) avoids challenges
associated with the original method, such as the inclusion of the
oral phase and the use of gastric lipase. The method can be used
to assess the endpoints resulting from digestion of foods by
analyzing the digestion products (e.g., peptides/amino acids,
fatty acids, simple sugars) and evaluating the release of
micronutrients from the food matrix. The whole protocol can be
completed in ~7 d, including ~5 d required for the determination
of enzyme activities.
structure and composition on human health has increasingly
involved simulating digestion in the upper gastrointestinal
tract. These simulations have used a wide range of different
conditions that often have very little physiological relevance,
and this impedes the meaningful comparison of results. The
standardized protocol presented here is based on an international
consensus developed by the COST INFOGEST network. The method is
designed to be used with standard laboratory equipment and
requires limited experience to encourage a wide range of
researchers to adopt it. It is a static digestion method that
uses constant ratios of meal to digestive fluids and a constant
pH for each step of digestion. This makes the method simple to
use but not suitable for simulating digestion kinetics. Using
this method, food samples are subjected to sequential oral,
gastric and intestinal digestion while parameters such as
electrolytes, enzymes, bile, dilution, pH and time of digestion
are based on available physiological data. This amended and
improved digestion method (INFOGEST 2.0) avoids challenges
associated with the original method, such as the inclusion of the
oral phase and the use of gastric lipase. The method can be used
to assess the endpoints resulting from digestion of foods by
analyzing the digestion products (e.g., peptides/amino acids,
fatty acids, simple sugars) and evaluating the release of
micronutrients from the food matrix. The whole protocol can be
completed in ~7 d, including ~5 d required for the determination
of enzyme activities.
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